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Mechanically Induced Bacterial Death Imaged in Real Time: A Simultaneous Nanoindentation and Fluorescence Microscopy Study

Overview of attention for article published in ACS Applied Materials & Interfaces, June 2020
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#46 of 17,592)
  • High Attention Score compared to outputs of the same age (98th percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

Mentioned by

news
5 news outlets
blogs
1 blog
twitter
216 tweeters
facebook
2 Facebook pages

Citations

dimensions_citation
11 Dimensions

Readers on

mendeley
30 Mendeley
Title
Mechanically Induced Bacterial Death Imaged in Real Time: A Simultaneous Nanoindentation and Fluorescence Microscopy Study
Published in
ACS Applied Materials & Interfaces, June 2020
DOI 10.1021/acsami.0c08184
Pubmed ID
Authors

Adrián del Valle, Joaquim Torra, Patricia Bondia, Caterina M. Tone, Patricia Pedraz, Virginia Vadillo-Rodriguez, Cristina Flors

Abstract

Mechano-bactericidal nanomaterials rely on their mechanical or physical interactions with bacteria and are promising antimicrobial strategies that overcome bacterial resistance. However, the real effect of mechanical versus chemical action on their activity is under debate. In this paper, we quantify the forces necessary to produce critical damage to the bacterial cell wall by performing simultaneous nanoindentation and fluorescence imaging of single bacterial cells. Our experimental setup allows puncturing the cell wall of an immobilized bacterium with the tip of an atomic force microscope (AFM) and following in real time the increase in the fluorescence signal from a cell membrane integrity marker. We correlate the forces exerted by the AFM tip with the fluorescence dynamics for tens of cells, and we find that forces above 20 nN are necessary to exert critical damage. Moreover, a similar experiment is performed in which bacterial viability is assessed through physiological activity, in order to gain a more complete view of the effect of mechanical forces on bacteria. Our results contribute to the quantitative understanding of the interaction between bacteria and nanomaterials.

Twitter Demographics

The data shown below were collected from the profiles of 216 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 30 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 20%
Student > Master 4 13%
Student > Ph. D. Student 4 13%
Student > Bachelor 3 10%
Lecturer 2 7%
Other 2 7%
Unknown 9 30%
Readers by discipline Count As %
Chemistry 4 13%
Engineering 4 13%
Biochemistry, Genetics and Molecular Biology 3 10%
Materials Science 3 10%
Physics and Astronomy 2 7%
Other 3 10%
Unknown 11 37%

Attention Score in Context

This research output has an Altmetric Attention Score of 195. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 August 2022.
All research outputs
#171,446
of 23,065,445 outputs
Outputs from ACS Applied Materials & Interfaces
#46
of 17,592 outputs
Outputs of similar age
#6,097
of 397,031 outputs
Outputs of similar age from ACS Applied Materials & Interfaces
#3
of 714 outputs
Altmetric has tracked 23,065,445 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 17,592 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 397,031 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 714 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.